chemiluminescence autoradiography ecl kit sc 2048 santa cruz biotechnology (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology chemiluminescence autoradiography ecl kit sc 2048 santa cruz biotechnology
Chemiluminescence Autoradiography Ecl Kit Sc 2048 Santa Cruz Biotechnology, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/santa+cruz+sc/Western+Blotting+Luminol+Reagent/pmc12795691-113-10-11
Average 96 stars, based on 3284 article reviews

chemiluminescence autoradiography ecl kit sc 2048 santa cruz biotechnology - by Bioz Stars, 2026-10

96/100 stars

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Blocking Assay:

Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
Article Snippet: .. Following blocking, tissues were submerged in 1x PBS containing 5% fetal bovine serum with primary anti-bodies against LYZ1FITC (1:200) (Dako: F037201), PCNA (1:200) (Santa Cruz: sc-56), GFP (1:100) (Cell Signaling: 295SS), MUC2 (1:200) (Abcam: ab272692), DCLK1 (1:200) (Cell Signaling: 62257S), ADAM17 (1:200) (Invitrogen: MA5-32572), RFP (1:200) (Rockland: 200-101-379), UEA1-Dylight 649 AR TI CL E IN P RE SS ARTICLE IN PRESS (1:200) (Vector: DL-1068), IL-17RA (1:200) (Abcam: ab180904), ChGA (1:200) (Santa Cruz: sc393941), I-FABP (1:200) (Santa Cruz: sc-374482) and ZO-1 (1:200) (Invitrogen: 61-7300) at 4 C overnight. .. The next day, slides were washed three times with 1x PBS, the sections were incubated for 30 minutes at room temperature with Alexa Fluor® 488 AffiniPureTM Goat Anti-Rabbit IgG (H+L) (1:200) (Jackson ImmunoResearch: 111-545-144), Alexa Fluor® 647 AffiniPureTM F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)(1:200) (Jackson ImmunoResearch: 111-606-144), Alexa Fluor® 488 AffiniPureTM F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (1:200) (Jackson ImmunoResearch: 115-546-003), or Alexa Fluor® 647 AffiniPureTM F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) (1:200) (Jackson ImmunoResearch: 705-606-147).

Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
Article Snippet: .. After blocking, organoids were incubated overnight at 4C in 1X PBS containing 5% BSA with primary antibodies against GFP (1:100) (Rockland: 600-901-215), Muc2 (1:200) (Santa Cruz: sc-515032), or ChGA (1:200) (Santa Cruz: sc-393941). .. The next day, organoids were washed with 1X PBS and then incubated for 30 minutes at 37C in 1X PBS containing 5% BSA with secondary antibodies Alexa Fluor® 488 AffiniPureTM F(ab')2 Fragment Donkey Anti-Chicken IgG (H+L) (1:100) (Jackson ImmunoResearch: 703-544-155) and Alexa Fluor® 647 AffiniPureTM F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (1:200) (Jackson ImmunoResearch: 715-605-150).

Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
Article Snippet: .. After blocking, organoids were incubated overnight at 4 C in 1X PBS containing 5% BSA with primary antibodies against GFP (1:100) (Rockland: 600-901-215), Muc2 (1:200) (Santa Cruz: sc-515032), or ChGA (1:200) (Santa Cruz: sc-393941). .. The next day, organoids were washed with 1X PBS and then incubated for 30 minutes at 37 C in 1X PBS containing 5% BSA with secondary antibodies Alexa Fluor® 488 AffiniPureTM F(ab')2 Fragment Donkey Anti-Chicken IgG (H+L) (1:100) (Jackson ImmunoResearch: 703-544-155) and Alexa Fluor® 647 AffiniPureTM F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (1:200) (Jackson ImmunoResearch: 715-605-150).

Incubation:

Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
Article Snippet: .. After blocking, organoids were incubated overnight at 4C in 1X PBS containing 5% BSA with primary antibodies against GFP (1:100) (Rockland: 600-901-215), Muc2 (1:200) (Santa Cruz: sc-515032), or ChGA (1:200) (Santa Cruz: sc-393941). .. The next day, organoids were washed with 1X PBS and then incubated for 30 minutes at 37C in 1X PBS containing 5% BSA with secondary antibodies Alexa Fluor® 488 AffiniPureTM F(ab')2 Fragment Donkey Anti-Chicken IgG (H+L) (1:100) (Jackson ImmunoResearch: 703-544-155) and Alexa Fluor® 647 AffiniPureTM F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (1:200) (Jackson ImmunoResearch: 715-605-150).

Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
Article Snippet: .. After blocking, organoids were incubated overnight at 4 C in 1X PBS containing 5% BSA with primary antibodies against GFP (1:100) (Rockland: 600-901-215), Muc2 (1:200) (Santa Cruz: sc-515032), or ChGA (1:200) (Santa Cruz: sc-393941). .. The next day, organoids were washed with 1X PBS and then incubated for 30 minutes at 37 C in 1X PBS containing 5% BSA with secondary antibodies Alexa Fluor® 488 AffiniPureTM F(ab')2 Fragment Donkey Anti-Chicken IgG (H+L) (1:100) (Jackson ImmunoResearch: 703-544-155) and Alexa Fluor® 647 AffiniPureTM F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (1:200) (Jackson ImmunoResearch: 715-605-150).

other:

Article Title: Phagocytic podosomes enable efficient uptake of Candida auris by primary human macrophages.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Arp2 Abcam ab49674; RRID: AB_867730 Lot:1004758-3 vinculin (clone hVin-1) Sigma-Aldrich V9264; RRID:AB_10603627 Lot:0000409222 CD11b/αM Integrin BD Pharmingen 550374; RRID:AB_393645 Lot:1277879 α-actinin Santa Cruz sc-17829; RRID:AB_626633 Lot:H0620 myosin If (B-5) Santa Cruz sc-376534; RRID:AB_11150122 Lot:D1917 MT1-MMP (MMP-14) Merck Millipore MAB3328; RRID:AB_570599 Lot:3974627 DNaseX (DNASE1L1) Abnova H0001774-M02; RRID:AB_534842 Lot:N220-4E8 Myosin IIA Sigma-Aldrich M8064; RRID:AB_260673 pTyr Santa Cruz sc-7020; RRID:AB_628123 Lot:G0822 cortactin BD Transduction Laboratories 610049; RRID:AB_397461 talin-1 Bio-RAD 97H6; PRID:AB_10671591 zyxin Invitrogen 39–6000; RRID:AB_2533422 Lot:299185R LSP1 AtlasAntibodies (Sigma) HPA019693; RRID:AB_1853387 Lot:6104845 WASp (B-9) Santa Cruz sc-13139; RRID:AB_628445 Lot:A1723 Anti-mouse-Abberior STAR RED Abberior STRED; RRID:AB_3068620 Alexa Fluor 647 (F ab’)2◦ ab. fragment goat anti rabbit IgG (H + L) Invitrogen A21246; RRID:AB_1500778 Lot:2936370 Alexa Fluor 568 secondary goat anti rabbit IgG (H + L) Invitrogen A21246; RRID: AB_143157 Lot:2936370 Alexa Fluor 488 secondary donkey anti rabbit IgG (H + L) Invitrogen A21069; RRID:AB_141416 Lot:2570539 Alexa Fluor 647 secondary goat anti mouse IgG (H + L) Invitrogen A21235; RRID:AB_2535804 Lot:3192624 Alexa Fluor 568 secondary donkey anti mouse IgG (H + L) Abcam Ab175472; RRID:AB_2636996 Lot:3192624 Alexa Fluor 568 secondary goat anti mouse IgG2a Invitrogen A21134; RRID:AB_1500825 Lot:2680411 Alexa Fluor 488 secondary donkey anti rabbit IgG (H + L) Invitrogen A1100; RRID: AB_2535792 Lot:3148303 CD11b/αM Integrin (D6XIN) Cell Signaling 49420; RRID:AB_2799357 Lot:7 CD16 (clone3G8) BD Pharmingen 555406; RRID:AB_395806 Lot:5113259 CD18/ß2 Integrin R&D Systems FAB1730R-100UG; RRID:AB_3647023 Lot:1793473 (Continued on next page) Cell Reports 45, 117167, April 28, 2026 13

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chemiluminescence autoradiography ecl kit sc 2048 santa cruz biotechnology (Santa Cruz Biotechnology)

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